Used Chromatography Equipment
Chromatography equipment separates mixtures into individual components based on differences in molecular properties. These systems use stationary and mobile phases to isolate, identify, and purify compounds ranging from small molecules to large biomolecules, such as proteins and antibodies.
Federal Equipment Company stocks chromatography systems and columns from Cytiva, Sartorius, GE Healthcare, Millipore, and other leading manufacturers. Inventory includes single-use and traditional chromatography systems, flash chromatography systems, supercritical fluid chromatography (SFC) equipment, and chromatography columns in various diameters and bed heights.
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Chromatography systems function by passing a mobile phase—liquid in most process applications, or gas in GC systems—through a stationary phase contained in a column or cartridge. Different compounds in the sample interact with the stationary phase at different rates, resulting in distinct elution times. This separation allows for purification of target molecules, removal of impurities, and analytical characterization of complex mixtures.
Modern chromatography systems include automated controls for flow rate, pressure, gradient formation, and fraction collection. Single-use systems eliminate cleaning validation between batches, while traditional stainless steel systems offer lower consumable costs for high-volume production. Key components include pumps, detectors (UV, conductivity, refractive index), fraction collectors, and columns packed with appropriate resins or media.
Federal Equipment Company offers chromatography equipment across multiple scales and technologies. Our inventory includes ÄKTA and AKTAready systems for biotechnology and pharmaceutical applications, process-scale purification systems, and flash chromatography units for rapid batch separations. Column diameters range from laboratory scale to production scale, with bed heights from 50 cm to over 80 cm. Equipment is available for inspection and can be refurbished or tested prior to shipment.
Applications and Industries
- Monoclonal antibody purification and polishing in biopharmaceutical manufacturing
- Protein and enzyme isolation for therapeutic and diagnostic applications
- Peptide purification following solid-phase synthesis
- Natural product extraction and compound isolation
- Vaccine production and viral vector purification
- Blood plasma fractionation for immunoglobulin production
- Small molecule API purification in pharmaceutical synthesis
- Botanical extract purification in extraction operations
- Quality control and analytical method development
- Process development and scale-up from bench to production
Specifications and Configurations
- System types: Single-use, traditional stainless steel, bench-top, pilot, and production scale
- Technologies: Liquid chromatography (LC), flash chromatography, supercritical fluid chromatography (SFC), gas chromatography (GC)
- Column diameters: Laboratory (less than 10 cm) through production scale (50+ cm)
- Bed heights: 20 cm to 100+ cm, depending on application
- Pressure ratings: Low pressure (up to 25 psi) through high pressure (870+ psi)
- Materials of construction: 316L stainless steel, glass, and single-use polymer assemblies
- Operating temperatures: Ambient to 200°F (93°C) depending on system
- Flow rates: 1 mL/min (analytical) to 1000+ mL/min (preparative and process scale)
- Controls: Manual, semi-automated, and fully automated with PLC integration
Why Buy Used Chromatography Equipment
Used chromatography systems typically cost 40-60% less than new equipment while delivering equivalent separation performance. This cost reduction is significant for facilities expanding purification capacity, equipping pilot plants, or validating new processes before committing to new equipment purchases.
Lead times for used chromatography equipment are measured in days or weeks rather than the months often required for new system delivery. This accelerated timeline supports faster project completion and helps facilities meet production schedules when capacity needs change unexpectedly.
Federal Equipment Company can provide inspection, refurbishment, and functional testing services for chromatography systems. These services verify operational status, replace worn components, and confirm system performance meets specifications before delivery.
